基于10X Geomics ChromiumTM动态微流控技术,利用Tn5转座酶酶切开放染色质,形成短片段,单细胞ATAC测序在表观基因组学水平上,揭示单细胞染色质的可及性问题,区分细胞异质性,获得开放染色质的位置、转录因子的结合位点、核小体的调控区域和染色质状态等信息,是单细胞表观遗传学的重要突破。
Alexandro E. Trevino et al. Cell. 2021 Sep
1. 丰富的样本制核经验,细胞核捕获率高达65%
2. 对培养的细胞系、血液、新鲜和冻存组织等样本均可实验(冻存组织限于脑和心脏)
3. 烈冰自主搭建分析云平台,一键实现数据分析,包括peak calling、聚类、TF-motif、细胞亚群、Marker基因和信号通路富集分析等
样本类型:
组织、血液、培养的细胞系、制备好的单细胞悬液
质量要求:
1. 细胞活性大于70%
2. 浓度为500-2000细胞/μl
3. 体积不小于200μl
4. 细胞培养基及缓冲液不能含Ca2+和Mg2+
5. 细胞体积小于40μm
Jason D, et al. Cell, 2018; Wenliang Wang et al. PNAS, 2020.
Darren A. Cusanovich, et al. Cell, 2018.
Jeffrey M., et al. Nature Biotechnology, 2019.
Jason D, et al. Cell, 2018.
左图为单细胞RNA-seq和ATAC-seq数据整合:通过整合单细胞转录组和ATAC数据,确定ATAC细胞类型注释的可靠性与一致性。
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