单细胞测序技术(Single Cell Sequencing)是在单个细胞水平上,揭示全基因组范围内的所有基因表达情况,包括鉴定组织细胞类型,反映不同样本间的细胞异质性和组织微环境。
【烈冰智造】于2021年9月发布了第一款用于单细胞测序的实验耗材——PanoCell®双通道微孔芯片;2022年9月推出了灵活度更高、兼容性更好的PanoCell®微孔芯片V2.0版。升级后的V2.0芯片将提供自研蜂巢板+BD平台原装进口单细胞分选试剂+华大T7超高通量测序仪的服务新方案!
1. 自主研发PanoCell微孔芯片,搭配BD Rhapsody单细胞平台+BD磁珠+BD全套原装试剂及protocol;
2. 具有丰富的、针对不同样本类型的单细胞悬液制备经验,如:外周血、细胞系、新鲜/冻存器官组织、肿瘤组织,确保细胞活性达到测序要求;
3. 拥有成熟的单细胞测序文库构建技术,一次性完成1000-10000个细胞的文库构建,真正测全组织中所有细胞类型,做到对样本中所有类型细胞的全面解析;
4. 具备完善的单细胞测序和实验质控流程,以及丰富的单细胞测序和数据分析经验,实现个性化、定制化数据分析服务。
样本类型:
组织、血液、培养的细胞系、制备好的单细胞悬液
注:若客户样本为组织,且无能力进行组织解离来获取单细胞悬液,烈冰将尽可能提供技术及实验上的帮助,但因不同类型样本的特异性,无法保证实验方法适用于所有类型组织。
质量要求:
1. 细胞活性大于70%
2. 浓度为500-2000细胞/μl
3. 体积不小于200μl
4. 细胞培养基及缓冲液不能含Ca2+和Mg2+
5. 细胞体积小于40μm
BD Rhapsody?单细胞分析系统的诞生基于 BD 在细胞生物学领域 40年的专业技术, 采用CytoSeq技术进行单细胞捕获。应用烈冰PanoCell微孔芯片(包含20W+的微孔(该数量级远大于Input细胞数量)),搭配BD平台磁珠+全套试剂+protocol,保证单孔中的单细胞捕获。同时避免了传统微流控系统中存在的概率碰撞影响捕获效率的问题,采用微孔捕获相对会有更好的捕获效率,保证Input细胞的全面使用。
Chen, et al.Nature Cell Biology, 2021 Jan;23(1):87-98.
注:左图为前列腺肿瘤组织细胞亚群鉴定t-SNE图展示;右图细胞亚群分群情况
Zhang et al., Glia. 2021 Mar;69(3):765-778.
Zhang, et al. Glia. 2021 Mar;69(3):765-778.
Zhang C, et al. J Immunother Cancer 2021;9:e002312.
Chen, et al. Nature Communication 2020; 11:5077
注:该图为不同cluster之间转录因子调控强度heatmap图
Chen, et al.Nature Cell Biology, 2021 Jan;23(1):87-98.
注:该图为不同cluster之间信号同理调控强度热图
Chen, et al.Nature Cell Biology, 2021 Jan;23(1):87-98.
注:细胞间状态转换的pesudotime轨迹图(左)、轨迹中细胞占比饼图 和 Heatmap图(右)
Chen, et al. Nature Communication 2020; 11:5077
注:左图:横坐标表示细胞类型,纵坐标表示细胞间通讯配受体关系,圆圈大小表示显著性差异水平,圆圈颜色越红表示细胞间通讯关系越强
Zhang C, et al. J Immunother Cancer 2021;9:e002312.
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